Differences in levelFms-Like Tyrosine Kinase-1 (sFlt-1), soluble Endoglin (s-Eng), and Placental Growth Factor (PIGF) between Early Onset Preeclampsia and Late Onset Preeclampsia

Lita Nafratilova; Yusrawati Yusrawati; Irza Wahi

doi:10.25077/jom.3.2.11-18.2018

Differences in levelFms-Like Tyrosine Kinase-1 (sFlt-1), soluble Endoglin (s-Eng), and Placental Growth Factor (PIGF) between Early Onset Preeclampsia and Late Onset Preeclampsia

DOI: http://dx.doi.org/10.25077/jom.3.2.11-18.2018

Author(s)

Lita Nafratilova (Andalas University)
Yusrawati Yusrawati
Irza Wahi (Andalas University)

Abstract

Early Onset Preeclampsia (EO-PE) is preeclampsia that develops before 34 weeks 'gestation, caused by intrinsic factors, while Late Onset Preeclampsia (LO-PE) is preeclampsia that develops after 34 weeks' gestation due to extrinsic and maternal factors. There is an increased production of antiangiogenic factors (sFlt-1, s-Eng and PIGF) contribute to pathophysiology of preeclampsia.This study aims to measure the difference of sFlt-1, sEng, PIGF levels between EO-PE and LO-PE. This was an observational study with cross sectional design conducted at Dr. M. Djamil, TK Hospital. III dr. Reksodiwiryo and Biomedical Laboratory FK Unand Padang from August 2017 to August 2018. The sample of this study were 26 severe preeclampsia women : 13 (EO-PE) and 13 (LO-PE), selected using consecutive sampling. Levels of sFlt-1, sEng, PIGF were examined using the enzyme-linked immunosorbent assay (ELISA) method. Statistical analysis was performed using unpaired t test and Mann-Whitney Test. Results shown that serum levels of sFlt-1 and sEng in (EO-PE) were 9.51 ± 0.71 ng / L, 1.44 ± 0.06 ng / mL, 5.79 ± 0.42 ng / mL while in PEAL it was 8, 89 ± 0.78 ng / mL, 1.35 ± 0.14 ng / mL, 6.72 ± 0.76. There were a significant difference with a value of p <0.05. The conclusion of this study is that the levels of sFlt-1 and sEng are higher in (EO-PE) than(LO-PE)and PIGF levels was lower in (EO-PE) compared to (LO-PE)

Keywords

sFlt-1, sEng, PIGF, PEAD, PEAL

Full Text:

PDF

References

Akbar, M, A, Herdiyantini, M, Aditiawarman. Perbandingan kadar soluble Endoglin (sEng) serum pada penderita Preeklamsia Tipe dini, Tipe lambat dan ibu hamil normal. Majalah Obsetri & Genekologi, Volume 25 No 1, pp. 10-15.2017.

Andalas, M. Harapan. Antagonis soluble tms-Like Tyrosine Kinase 1 (sFlt-1) dan soluble endogline (sEng) pada preeklamsia. JIK, Volume 1, pp. 1-9. 2010.

Berthold .H. Placental Origins of Preeclampsia. In :Challenging the Current Hypothesis. American Heart Association. 2008.

Cheng M, He P, Fu J. “The relationship between circulating tissue transglutaminase, soluble fms-like tyrosine kinase-1, soluble endoglin and vascular endothelial growth factor in pre-eclampsia. Journal of human hypertension . 30, 788-793. 2016.

Costa, FS. Murthi, P. Keogh, R. Woodrow, N. Early Screening for Preeclamsia. Melbourne-Australia :Rev Bras Ginecol Obstet. 2011 ; 33 (11) : 367-75. 2011.

Dinas Kesehatan Kota Padang. Profil Kesehatan Kota Padang tahun 2016. Padang : Dinas Kesehatan Kota Padang. 2016.

Ekapatria, C., Sabarudin, U. & Sasotya, S. Placental Growth Factor Level in Lower in early-Onset Preeclamsia, while Tumor Necrosis factor Alpha Level Does not Show any Difference between Early and late Onset Preeclampsia. Maj Obstet Ginekol Indonesia,Volume 36 No 4, pp. 36-4 : 181-4. 2012.

Gulec UK, Ozgunen FT, Buyukkurt S, Guzel AB, Urunsak IF, DemirSC,et al. Comparison of clinical and laboratory findings in early and late onset preeclampsia.TheJournalof Maternal-Fetal& Neonatal Medicine. 26-(12):1228-33.2013.

Hirashima C, Ahkuchi A, Matsubara S, Suzuki H, Takahashi K, Usui R, Suzuki M. “ Alteration of serum soluble endoglin levels after the onset of preeclampsia is more pronounced in women with early-onset. Hipertens Res.Vol 31, No 8. 2008

Lok, CA ; An, Boing ; IL Sargent, et al.Circulating Platelet-derived and placental-derived microparticle expose flt-1 in preeclampsia. Reproduction Scince, Volume 15, pp. 1002-1010. 2008.

Mbah AK, Kornosky JL, Kristensen S, Agust EM, Alo AP, Marty PJ, et al. Super Obesity and Risk for early and late preeclamsia. BJOG. 117 : 997-1004. 2010.

Perales A, Delgado JL, Calle M. De la, Hernandez Garcia, Escudero A.I, Campillos J.M, et al. sFlt-1/PIGF for prediction of early-onset pre-eclampsia : Steps (Study of early pre-eclampsia in Spain). Ultrasound Obstet Gynecol. 50 : 373-382. 2017.

Phipps, E.,Prasanna, D., Brima., W. & Jim, B., Preeclampsia : Updates in Pathogenesis, Definitions, and Guidelines, Clin J Am Soc Nephrol, Volume 11, pp. 1102-1113.2016.

Powe CE, Levine RJ, Karumanchi SA. (2011). “Preeclampsia, A Disease of The Maternal Endothelium: The Role of Anti-Angiogenic Factors and Implications for Later Cardiovascular Disease. Howard Hughes Medical Institute. 21:123 (24). 2011.

Rahmi, L., Herman, B & Yusrawati. Perbedaan Rerata Kadar Soluble fms-like Tyrosine Kinase-1 (sFlt-1) Serum pada penderita Early Onset,Late Onset Preeklamsia Berat/Eklamsia dan Kehamilan Normal. Jurnal Kesehatan Andalas, p.5 (1). 2016.

Raymon D, Peterson E.“A Critical Review of Early-Onset and Late-Onset Preeclampsia. Obstetry Gynecol.66(8): 497-506. 2011.

Reslan OM, Khalil RA. Molecular And Vascular Targets in The Pathogenesis and Management of The Hypertension Associated With Preeclampsia. Cardiovascular Hematol Agents Med Chem. 8(4): 204–226. 2010.

Rezi Elma, Yusrawati, Iryani Detty. Perbedaankadar Soluble endoglin Pada Preeklamsi Awitan Dini (PEAD) dengan Pre eklamsi awitan lambat (PEAL). Jurnal Kesehatan Andalas. 2017.

RSUP Dr.M.Jamil. Data Medical Record RSUP Dr. M. Djamil Padang. 2017Salan, Y. Biomarker Terkini Dalam Usaha Memperdiksi Preeklamsia. Berkala Kedokteran,Volume 13, pp. 119-128.2017

Sanchez-Aranguren LC, Prada CE., Riano-Medina CE, Lopez M. “Endothelial Dysfunction and Preeclampsia” : Role Of Oxidative Stress. Physiol : 5:372. 2014.

Sinuraya Rano K, Nisa Hidayatun, Lokajaya Trifena, Puri Tri NS. Biomarker PIGF/sFlt-1 sebagai Pendeteksi dini Preeklamsia. Jurnal Farmasi Klinik Indonesia. Vol.6 No. 2, hlm 123-134. 2017.

Soto, Eleazar ; Romeo, Roberto ; Kusnovic, Pedro, Juan ; Giovanna ; Hussein, Youssef : yeo, Lami : Hassan, S; Kim, Jai, Choy; Chaiworapongsa, Yinnakorn. Late-Onset Preeclamsia is Associated With An Imbalance of Angiogenic and Anti-Angiogenic Factror in Patiens With and Without Placental Lesions Consistent With Maternal Underperfusion. J. Matern Fetal Neonatal Med, pp. 498-507. 2012.

SUPAS. Profil Penduduk Indonesia Hasil SUPAS 2015, Jakarta : Badan Pusat Statistik. 2015

Villa, PM. Hamalainer, E. Maki, A. Raikkonen, K. Pesonen, A. Taipale, P. et al. Vasoactive agents for the prediction of early and late-onset preeclamsia in a high-risk cohort. BMC Pregnancy and Childbirth. Volume 13 : 110. 2013.

Vinayagam V, Bobby Z, Habeebullah S, Latha CH, Bharadwaj S.Maternal and cord bloodplasma sEngdanTGF-β1 in patients with hypertensive disorders of pregnancy: A pilot study in a south indian population.Journal of clinical and diagnostic research:vol 11(3): QC32-QC3. 2017.

WHO. Maternal Mortaliti: www.who.int/mwdiacentre/factsheets/fs348/e.2016

Wikstrom AK,Larsson, A. Eriksson, UJ. Nash, P. Norden-Lindeberg, S. Olovsson, M. Placental growth factor and soluble fms-like tyrosine kinase-1 in early onset and late onset preeclamsia. Obstetri and Gynecology. June : 109 (6) : 1368-74. 2007

Wibowo, Noroyono; Irwinda, Rima; Frisdiantiny, Edwina ; Karkata, Kornes ; Mose, c et al., [Online] Available at: or.id/publish/download/pnpk-dan-ppk [Diakses 6 11 2017].

Yusrawati. Perantakikdiastolikarteriuterinasebagaifaktorrisikodanperbedaanresistensi insulin, adma, hs-crp dan adiponektinantarapreeklamsiaawitandini dan preeklamsiaawitanlambat. Masters thesis, universitas andalas. 2015