Comparison of Sensitivity of Salmonella Typhi Bacteria Isolate Tifoid Fever Patients And Pure Culture To Some Antibiotics In Laboratory



DOI: https://doi.org/10.25077/jom.4.1.92-99.2019


Author(s)

Norma Farizah Fahmi (Airlangga University)
Lelly Aprilia Vidayati (Ngudia Husada Madura High School of Health Sciences)
Hamimmatus Zainiyah
Nailufar Firdaus

Abstract


Typhoid fever is one of the infectious diseases which can cause many problems in Indonesia and other developing countries. This fever occurs as a result of infections triggered by Salmonella typhi bacteria. The growth of Salmonella typhi can be inhibited using antibiotics. This study aims at investigating whether there is a difference in the sensitivity test of  Salmonella typhi bacteria in an isolate of patients with typhoid fever and pure culture in a laboratory on some antibiotics.

Salmonella typhi bacteria were isolated typhoid fever suspects at one of the hospitals in Surakarta. Pure cultures of Salmonella typhi bacteria were obtained from Microbiological Laboratory of Setia Budi University. Sensitivity test of antibiotics on Salmonella typhi bacteria used diffusion method. Data on antibiotics of inhibition zone diameter (mm) of antibiotics were analyzed statistically using the Two-Way Anova test.

The research results demonstrate that the sensitivity test on Salmonella typhi bacteria in an isolate of patients with typhoid fever shows resistance (R) towards amoxicillin and sensitivity (S) towards trimethoprim, chloramphenicol, gentamicin, ciprofloxacin. Meanwhile, pure culture shows sensitivity (S) towards trimethoprim, chloramphenicol, gentamicin, amoxicillin, ciprofloxacin. The diameter of the inhibition zone of the patient isolate is smaller than that of pure culture.


Keywords


sensitivity test, Salmonella typhi, antibiotics

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References


Cita, Y.P. 2010. Salmonella Typhi Bacteria and Typhoid Fever. Journal of Public Health, 6 (1).

Darmawati. 2009. Genetic Diversity of Salmonella typhi. Health Journal, 2 (1).

Erviani, A. 2013. Multidrug Analysis of Resistance to Antibiotics in Salmonella typhi With Multiplex PCR Technique. Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University, 1 (1): 51-60.

Masriadi and Susniati. 2013. Factor Related to Typhoid Fever Incident in Salewangan Regional General Hospital. Makassar: Epidemiology Department, Tamalatea College of Health Sciences, Makassar.

Shulman, S.T., Phair, J.P., and Sommers, H.M. 1994. Basic Biological and Clinical Infection. Fourth Edition. Yogyakarta: Gadjah Mada University Press.

Sopandi, T., and Wardah. 2014. Food microbiology. Yogyakarta: C.V Andi Offset.

Sulistianingsih, R., Dirk Y.P., and Waworuntu, L.V. 2014. Sensitivity of Antibiotics to Bacteria Isolated from Diabetic Ulcers in Abepura Hospital, Jayapura City. JOURNAL OF PAPUA BIOLOGY, 6 (2): 53-59.

Tjay, T.H., and Rahardja, K. 2002. Important Medicines: Efficacy, Use and Side Effects. Edition VI. Jakarta: Publisher PT. Elex Media Komputindo.

Yuliana, A. 2015. Sensitivity Test of Levofloxacin Antibiotics in the Market Against Salmonella Thyphosa Bacteria ATCC 2401. Bakti Tunas Husada Health Journal, 14 (1).

Zulkoni, A. 2011. Parasitology for Nursing, Public Health and Environmental Engineering. Yogyakarta: Nuha Medika.


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Copyright (c) 2019 Norma Farizah Fahmi

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Published by:
Undergraduate Program of Midwifery
Faculty of Medicine - Universitas Andalas - Indonesia
Faculty of Medicine, Universitas Andalas

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.